Cellular Respiration Yeast Lab Report
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The objective of this experiment was to measure how much oxygen is consumed when the yeast is respiring. In other words, to measure how much oxygen gets reduced by the yeast. Knowing the amount of oxygen consumed is an important part of the experiment because the amount of oxygen consumed can be used to determine how much energy is being produced by the yeast. Oxygen consumption can be measured using an oxygen sensor.
For each tube with added glucose, the temperature of the tube was measured and a thermometer was used to measure the temperature of the yeast mixture. Before measuring the CO2 readings, the CO2 sensors were put into the chamber. For the yeast mixture with added glucose, the CO2 readings were recorded for four minutes. After the four-minute period, the yeast mixture was incubated in the two-degree Celsius water bath for ten minutes and the CO2 readings were recorded for four minutes. The temperature of the mixture was measured and a thermometer was used to measure the temperature of the yeast mixture.
In this test, all the tubes with added yeast and glucose were incubated at the same time in the four-degree Celsius water bath for ten minutes. The temperature of the tube with added glucose was measured using a calibrated thermometer. These four tubes were then removed and the rest of the tubes, two from each temperature, were incubated in the next highest temperature water bath. The CO2 sensors were then set up and the CO2 readings were recorded for four minutes. After the four minutes, the CO2 sensors were removed and the yeast was incubated in the two-degree Celsius water bath for ten minutes. The temperature of the tubes from this bath were measured using a calibrated thermometer. After the ten-minute incubation period, the CO2 readings were recorded for a fourth time.
The overall setup was repeated, but this time varying the incubation temperature. For each yeast mixture, three separate tubes were incubated for ten minutes in the four-degree Celsius water bath, two tubes were incubated in the 30-degree Celsius bath, and two tubes were incubated in the 50-degree Celsius water bath. After the ten-minute incubation, the CO2 sensors were set up to record the CO2 values for four minutes. The CO2 readings were recorded for each tube every six times per minute and every four minutes. The CO2 readings were recorded until the CO2 sensors reached a steady reading.
The chemical reactions that take place in the cell are typically so specific that it is not possible for the cell to use anything but a molecule that is specifically made for that reaction. For example, the two molecules, ATP and ADP, are formed in a very specific reaction and cannot be substituted. When ATP is broken down by cellular respiration, only glucose can be used because the enzyme that breaks down ATP is specific for glucose.
Note: We are going to use the equation for cellular respiration above that we wrote on the board. We need to calculate the amount of ATP produced from a single molecule of glucose. So, to do this, we need to know how many molecules of ATP are produced from one molecule of glucose. The equation for the number of ATP molecules produced for each glucose molecule is as follows: 827ec27edc