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The ductus epididymis or epididymal duct (ED) is a special tube in the male reproductive system which connects the seminal vesicle to the excurrent duct of the testis. The ED is a connecting region where the sperms are matured and finally stored. In this video, we will see the microscopic structure of the epididymal ducts of mouse, including the epididymis and lumen contents. We will see the developmental process of the epididymal ducts and, as well, the sperm maturation process.
In the developing Mllerian duct of chicken embryos, we identified transcripts of genes previously implicated in duct specification, invagination and elongation (Supplementary Figure 1). In addition, a large number of differentially expressed novel genes were identified. As duct development progressed, an increasing number of genes were up-regulated compared to the E4.5 posterior mesonephros (control in static comparisons). Nine hundred six genes were differentially expressed in ducts compared to the control, while 188 genes showed differential expression dynamically across the three stages of duct development (E4.5 anterior vs E5.5 vs E6.5) (Fig. 1). Gene ontology analysis showed that specific developmental pathways were enriched during duct formation. These included pathways associated with cell adhesion, cell migration, cell proliferation, negative regulation of apoptosis, G-protein coupled receptor signaling, the extracellular matrix and WNT and ERK signaling. This is consistent with the cellular behaviour that occurs during duct morphogenesis, which involves proliferation, migration, interaction with the extra-cellular matrix, and epithelial-mesenchyme cross-talk [43].
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